中华临床医师杂志(电子版) 2017年2月,11卷3期

基础论著

银杏叶提取物对缺氧条件下人视网膜色素上皮细胞增殖和迁移的影响

王超1 朱秀英1 张素红1 贾延磊1 张晓梅2

277101 山东省,枣庄市立医院眼科1;150001 哈尔滨医科大学附属第一医院眼科医院2
张晓梅,Emal: zhangxm667@163.com

摘要:  目的 研究银杏叶提取物(EGB)对缺氧条件下人视网膜色素上皮(RPE)细胞的增殖和抑制的影响,探讨其作用机制。方法 0、5、10、50、100 mmol/L的EGB分别作用于缺氧条件下RPE细胞24、48、72 h,CCK-8法检测RPE细胞的增殖抑制率;CCK-8法检测缺氧条件下RPE细胞的增殖情况;细胞划痕实验检测RPE细胞的迁移能力。结果 不同浓度的EGB在不同时间对缺氧条件下RPE细胞的增殖抑制率不同,浓度为100 mmol/L的EGB在24、48、72 h对缺氧下RPE细胞的增殖抑制率作用分别为(71.10±27.13)%、(86.67±13.10)%、(97.02±11.35)%。在EGB浓度为100 mmol/L条件下与对照组比较差异均有统计学意义(均P<0.01);在缺氧时间为72 h时EGB浓度分别为5、10、50、100 mmol/L,RPE细胞增殖抑制率分别为(58.00±26.77)%、(74.26±10.07)%、(74.47±20.41)%、(97.02±11.35)%。在作用时间为72 h条件下与对照组比较差异均有统计学意义(均P<0.01);通过CCK-8法检测24、48、72 h缺氧组与对照组OD值差异均有统计学意义(分别为:t24 h=2.833,P<0.05;t48 h=2.576,P<0.05;t72 h=2.535,P<0.05),随缺氧时间增长,RPE细胞的增殖能力逐渐增强;在缺氧条件下EGB作用时细胞移行的速度比单纯缺氧时细胞移行速度明显减慢。结论 EGB可以有效地抑制缺氧条件下RPE细胞的增殖,并可抑制RPE细胞迁移,可能为增殖性玻璃体病变的研究和治疗提供一定的理论依据。

关键词:视网膜色素上皮; 细胞增殖; 细胞运动; 银杏叶提取物

哈尔滨市科技创新人才研究专项资金项目(2011RFXYS055)

Effect of EGB on the proliferation and migration in the hypoxia of human retinal pigment epithelium cells

Wang Chao1, Zhu Xiuying1, Zhang Suhong1, Jia Yanlei1, Zhang Xiaomei2

1Department of Ophthalmology, the Made Hospital of Zaozhuang, Zaozhuang 277101, China; 2Department of Ophthalmology, the First Affiliated Hospital of Haerbin Medical University, Haerbin 150001, China
Zhang Xiaomei, Email: zhangxm667@163.com

Abstract:  Objective To investigate the mechanism and effects of EGB on the proliferation and migration in the hypoxia of human retinal pigment epithelium (RPE) cells. Methods The 0 mmol/L, 5 mmol/L, 10 mmol/L, 50 mmol/L and 100 mmol/L EGB were used to treat RPE for 24 hours, 48 hours and 72 hours respectively. CCK-8 was used to detect the inhibiting rate of cell proliferation in the hypoxia; CCK-8 was used to detect cells proliferation in the hypoxia; Migration rate was measured by wound healing assay. Results The inhibiting rate of cell proliferation in the hypoxia was different in different times and different concentrations, the most significant inhibitory rate were found at 72 hours after RPEs exposed to 100 mmol/L group with statistical meaning (P<0.05); With the extension of time of hypoxia, the most cell proliferation was found at 72 hours with statistical meaning (P<0.05); The number of the cells migration of EGB in hypoxia was less than no EGB in hypoxia. Conclusion EGB can inhibit the proliferation and migration of RPEs and may provide some theoretical evidence for prevention of proliferative vitreoretinopathy (PVR) in clinic.

Keywords:Retinal pigment epithelium; Cell proliferation; Cell movement; EGB

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(编辑:梁雷 收稿日期:2015-10-11)