中华临床医师杂志(电子版) 2017年2月,11卷3期

临床论著

Musashi-1、细胞转录因子4、尾侧型同源转录因子2在贲门腺癌组织中的表达及临床意义

张琳璐1 褚传莲2 李芳2 张艳敏1 马晓丽3 邹蕊霞2 孙岳婷2 周晓艳2 左芳2

261053 山东潍坊,潍坊医学院临床学院消化内科1;250013 济南,山东大学附属济南市中心医院保健消化科2,中心实验室
褚传莲,Email: chuchucl@163.com

摘要:  目的 研究人贲门腺癌中Musashi-1、细胞转录因子4(Tcf4)、尾侧型同源转录因子2(Cdx2)的表达及临床意义。方法 采用免疫组化法检测贲门腺癌、癌旁组织、正常贲门组织中Musashi-1、Tcf4、Cdx2的表达情况,采用Western blot检测贲门腺癌及癌旁组织中Musashi-1、Tcf4、Cdx2的表达,并分析其表达与临床病理特征、危险因素的关系。结果 在正常贲门上皮、癌旁组织、贲门腺癌中,Musashi-1阳性表达率分别为20.0%、45.0%、79.3%(P<0.05),Tcf4的阳性表达率分别为20.0%、40.0%、75.9%(P<0.05),Cdx2的阳性表达分别为20.0%、60.0%、37.9%(P>0.05);Western blot结果显示Musashi-1、Tcf4在癌组织中的表达高于癌旁组织,Cdx2在癌旁组织中的表达高于癌组织;Musashi-1与Tcf4在贲门腺癌组织中呈正相关(r=0.662,P<0.05),Tcf4与Cdx2在癌旁组织中呈弱相关(r=0.375,P<0.05),Musashi-1与Cdx2在癌组织中无明显相关(P>0.05);Musahi-1、Tcf4、Cdx2的表达主要与肿瘤组织分化程度有关(P<0.05);Musahi-1、Tcf4、Cdx2的表达与吸烟、幽门螺杆菌感染、胃食管反流病密切相关。结论 Musashi-1、Tcf4、Cdx2可能与贲门腺癌的发生、发展相关,三者在贲门腺癌中表达与组织分化程度有关。远离危险因素可能会减少贲门腺癌的发生。

关键词:Musashi-1; 细胞转录因子4; 尾侧型同源转录因子2; 贲门腺癌; 肿瘤干细胞

山东省自然科学基金(ZR2016HM25)

Expression and clinical significance of Musashi-1, Tcf4 and Cdx2 in cardia adenocarcinoma

Zhang Linlu1, Chu Chuanlian2, Li Fang2, Zhang Yanmin1, Ma Xiaoli3, Zou Ruixia2, Sun Yueting2, Zhou Xiaoyan2, Zuo Fang2.

1Department of Gastroenterology, Clinical Institue of Weifang Medical University, Weifang 261053, China; 2Department of Gastroenterology, 3Central Laboratory, Jinan Central Hospital of Shandong Univer
Chu Chuanlian, Email: chuchucl@163.com

Abstract:  Objective To explore the expression and clinical significance of Musashi-1, transcription factor 4 (Tcf4) and caudal type homeobox transcription factor 2 (Cdx2) in cardia adenocarcinoma (CA). Methods The expression of Musashi-1, Tcf4 and Cdx2 in normal cardia tissue, para-carcinoma issue and CA were detected by immunohistochemistry. The expressions of Musashi-1, Tcf4 and Cdx2 protein in para-carcinoma issues and CA were measured by Western blot. The clinical pathological parameters and the relationship between the expression of Musashi-1, Tcf4 and Cdx2 and risk factors of CA including tobacco, H. pylori infection and gastroesophageal reflux disease (GERD) were analyzed. Results In normal cardiac tissue, para-carcinoma tissue and CA, the positive rates of Musashi-1 was 20.0%, 45.0%, 79.3% (P<0.05), respectively. The positive rates of Tcf4 was 20.0%, 40.0%, 75.9% (P<0.05), respectively. The positive rates of Cdx2 was 20.0%, 60.0%, 37.9% (P>0.05), respectively. The protein expressions of Musahi-1 and Tcf4 in CA were significantly higher than those in para-carcinoma issues, Cdx2 expression in CA were significantly lower than those in para-carcinoma issues. The expression of Musashi-1, Tcf4 and Cdx2 were closely related to histological grading (P<0.05). The positive expression of these biomarkers had relationships between Musashi-1 and Tcf4 in CA, between Tcf4 and Cdx2 in para-carcinoma tissue (r=0.662, P<0.05, r=0.375, P<0.05, respectively). In addition, Musashi-1 and CDX2 had no relevance in CA (P>0.05). There were positive correlations between the expression of Musashi-1, Tcf4 and Cdx2 and the risk factors of CA including tobacco, H. pylori infection and GERD. Conclusions The expression of Musashi-1, Tcf4 and Cdx2 may be involved in the initiation, development of CA, and are associated with the degree of differentiation of the CA, which can be used for the early diagnosis of precancerous lesions. Away from the risk factors could reduce the occurrence of CA and precancerous.

Keywords:Musashi-1; Transcription factor 4; Caudal type homeobox transcription factor 2; Cardia adenocarcinoma; Tumor stem cell

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(编辑:马超 收稿日期:2016-12-08)