中华医学会系列杂志
Chinese Journal of Clinicians ISSN 1674-0785 CN 11-9147/R 联系我们网站地图帮助中心
期刊导读
期刊存档

您的位置:首页>> 文章摘要

银杏叶提取物对缺氧条件下人视网膜色素上皮细胞增殖和迁移的影响

王超1 朱秀英1 张素红1 贾延磊1 张晓梅2

277101 山东省,枣庄市立医院眼科1;150001 哈尔滨医科大学附属第一医院眼科医院2

张晓梅,Emal: zhangxm667@163.com

哈尔滨市科技创新人才研究专项资金项目(2011RFXYS055)

摘 要:  目的 研究银杏叶提取物(EGB)对缺氧条件下人视网膜色素上皮(RPE)细胞的增殖和抑制的影响,探讨其作用机制。方法 0、5、10、50、100 mmol/L的EGB分别作用于缺氧条件下RPE细胞24、48、72 h,CCK-8法检测RPE细胞的增殖抑制率;CCK-8法检测缺氧条件下RPE细胞的增殖情况;细胞划痕实验检测RPE细胞的迁移能力。结果 不同浓度的EGB在不同时间对缺氧条件下RPE细胞的增殖抑制率不同,浓度为100 mmol/L的EGB在24、48、72 h对缺氧下RPE细胞的增殖抑制率作用分别为(71.10±27.13)%、(86.67±13.10)%、(97.02±11.35)%。在EGB浓度为100 mmol/L条件下与对照组比较差异均有统计学意义(均P<0.01);在缺氧时间为72 h时EGB浓度分别为5、10、50、100 mmol/L,RPE细胞增殖抑制率分别为(58.00±26.77)%、(74.26±10.07)%、(74.47±20.41)%、(97.02±11.35)%。在作用时间为72 h条件下与对照组比较差异均有统计学意义(均P<0.01);通过CCK-8法检测24、48、72 h缺氧组与对照组OD值差异均有统计学意义(分别为:t24 h=2.833,P<0.05;t48 h=2.576,P<0.05;t72 h=2.535,P<0.05),随缺氧时间增长,RPE细胞的增殖能力逐渐增强;在缺氧条件下EGB作用时细胞移行的速度比单纯缺氧时细胞移行速度明显减慢。结论 EGB可以有效地抑制缺氧条件下RPE细胞的增殖,并可抑制RPE细胞迁移,可能为增殖性玻璃体病变的研究和治疗提供一定的理论依据。

关键词:视网膜色素上皮; 细胞增殖; 细胞运动; 银杏叶提取物

Effect of EGB on the proliferation and migration in the hypoxia of human retinal pigment epithelium cells

Wang Chao1, Zhu Xiuying1, Zhang Suhong1, Jia Yanlei1, Zhang Xiaomei2

1Department of Ophthalmology, the Made Hospital of Zaozhuang, Zaozhuang 277101, China; 2Department of Ophthalmology, the First Affiliated Hospital of Haerbin Medical University, Haerbin 150001, China

Zhang Xiaomei, Email: zhangxm667@163.com

Abstract:  Objective To investigate the mechanism and effects of EGB on the proliferation and migration in the hypoxia of human retinal pigment epithelium (RPE) cells. Methods The 0 mmol/L, 5 mmol/L, 10 mmol/L, 50 mmol/L and 100 mmol/L EGB were used to treat RPE for 24 hours, 48 hours and 72 hours respectively. CCK-8 was used to detect the inhibiting rate of cell proliferation in the hypoxia; CCK-8 was used to detect cells proliferation in the hypoxia; Migration rate was measured by wound healing assay. Results The inhibiting rate of cell proliferation in the hypoxia was different in different times and different concentrations, the most significant inhibitory rate were found at 72 hours after RPEs exposed to 100 mmol/L group with statistical meaning (P<0.05); With the extension of time of hypoxia, the most cell proliferation was found at 72 hours with statistical meaning (P<0.05); The number of the cells migration of EGB in hypoxia was less than no EGB in hypoxia. Conclusion EGB can inhibit the proliferation and migration of RPEs and may provide some theoretical evidence for prevention of proliferative vitreoretinopathy (PVR) in clinic.

Key words:Retinal pigment epithelium; Cell proliferation; Cell movement; EGB

评论   收藏 全文阅读: FullText |

文献标引:王超1 朱秀英1 张素红1 贾延磊1 张晓梅2.银杏叶提取物对缺氧条件下人视网膜色素上皮细胞增殖和迁移的影响[J/CD].中华临床医师杂志:电子版,2017,11(3):438.

参考文献:
  [1] 曹鎏, 马健, 袁志兰, 戈应滨. 金雀异黄素对培养的人RPE细胞诱导凋亡作用的研究[J]. 眼科新进展, 2010, 12(12): 1123-1126.

  [2] Esser P, Heimann K, Barta K, et al. Apoptosis in proliferative vitreoretinal disorders: possible involvement of TGF- induced RPE cell apoptosis[J]. Exp Eye Res, 1997, 65(2): 365-378.

  [3] 李端庄, 梁轩伟, 严灿荣, 等. 银杏叶提取物对兔缺血在灌注损伤后视网膜线粒体功能的保护作用[J]. 眼科新进展, 2004, 8(24): 280-282.

  [4] 朱洁, 王雨生, 张金生. 缺氧对培养人视网膜色素上皮细胞线粒体酶活性的影响[J]. 眼科新进展, 2005, 4(25): 105-108.

  [5] Ghazi-Nouri SM, Ellis JS, Moss S, et al. Charter is DG. Expression and localization of BDNF, NT 4 and TrkB in proliferative vitreoretinopathy[J]. Exp Eye Res, 2008, 86(5): 819-827.

  [6] Hinton DR, He S, Jin ML, et al. Novel growth factor involved in the pathogenesis of proliferative vitreoretinopathy[J]. Eye, 2002, 16(4): 422-428.

  [7] Weller M, Wiedemann P, Heimann K. Proliferative vitreoretinopathy-is it anything more than wound healing at the wrong place?[J]. Int Ophthalmol, 1990, 14(2): 105-117.

  [8] Collisond J, Tovellve, Coombesl J. Potentiation of ATP induced Ca2+ mobilization in human retinal pigment epithelial cells[J]. Exp Eye Res, 2005, 80(4): 465-475.

  [9] 焦明菲, 颜华. 增生性玻璃体视网膜病变的药物治疗[J]. 眼科研究, 2010, 28(4): 381-384 .

  [10] Maitra I, Marcocci I, Droy-Lefaix MT. Peroxyl radical scavenging activity of Ginkgo biloba extract GBE761[J]. Biochem Pharmacol, 2001, 26(8): 1649-1655.

  [11] Liu HL, Feng GZ, Wu L. The effects of rapamycin on lens epithelial cell proliferation, migration, and matrix formation: An in vitro study[J]. Mol Vision, 2010, 16(1): 1646-1653.

  [12] 张晓梅, 李钟睿, 穆华. 金纳多对高糖所致视网膜Müller细胞合成谷胱甘肽减少的保护作用[J]. 眼科研究, 2007, 25(10): 758-761.

  [13] Cogo A, Napolitano G, Michoud MC, et al. Effect of hypoxia on rat airway smooth muscle cell proliferation[J]. J Appl Physiol, 2003, 94(9): 1403-1409.

  [14] Singh S, Zheng JJ, Peiper SC, et al. Gene expression profile of ARPE-19 during repair of monolayer[J]. Graefes Arch Clin Exp Ophthalmol, 2001, 239(1): 946-951.

  [15] Yoshida A, Elner SG, Bian ZM, et al. Induction of interleukin-8 in human retinal pigment epithelial cells after denuding injury[J]. Br J Ophthalmol, 2001, 85(4): 872-876.

基础论著

银杏叶提取物对缺氧条件下人视网膜色素上皮细胞增殖和迁移的影响

王超1 朱秀英1 张素红1 贾延磊1 张晓梅2. .中华临床医师杂志:电子版 2017;11(3):438.

摘要 FullText 评论 收藏

外周血清素对离体Sprague-Dawley大鼠成骨细胞的影响

崔晓鹏1,2 韩树峰2 卢向东2 孙海飚2 贾二龙2 梁文锴2 . .中华临床医师杂志:电子版 2017;11(3):442.

摘要 FullText 评论 收藏

敲低卷曲螺旋结构域蛋白34表达对膀胱癌T24细胞增殖、迁移和侵袭能力的影响

巩艳青 宁向辉 李学松 周利群 郭应禄. .中华临床医师杂志:电子版 2017;11(3):452.

摘要 FullText 评论 收藏